Lab Standard Operating Procedures

General Lab Protocols

  1. Ordering Non-Catalog Items
  2. Ordering through Sunrise
  3. Ordering via Purchase Order
  4. Heart-on-Chip Training Presentation

Equipment Protocols

  1. SOP for Autoclaving Pipette Tips
  2. SOP for Operating Biosafety Cabinet – Level 2

Technical Protocols

  1. Biotium SOP for Live Dead Assay
  2. BMSEED User Manual for the Electrophysiology Module
  3. BMSEED User Manual for the Electrophysiology Module using the IntanRHX Software
  4. BMSEED User Manual for the Temperature Controller
  5. OD versus Concentration for Gold Nanorods
  6. Optimization of Surface Treatment of sMEAS for seeding hiPSC-CMs
  7. SOP for Actin Staining
  8. SOP for Agarose Microwells – Casting, Equilibrating, and Seeding 3D Petri Dish
  9. SOP for Cancer Invasion Platform
  10. SOP for Cardiac Cell Isolation
  11. SOP for Culturing MDA 231 Cells
  12. SOP for Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/beta-catenin signaling
  13. SOP for Fabricating Hydrogels
  14. SOP for Freezing hCF
  15. SOP for GelMA Synthesis from Porcine Gelatin
  16. SOP for Gold Nanorod Synthesis
  17. SOP for Gold Nanosphere Synthesis
  18. SOP for Gold Nanowire Synthesis
  19. SOP for hiPSC Culture, Differentiation, and Preparation
  20. SOP for Immunostaining of Microfluidic Devices
  21. SOP for IMR90-4 hiPSC Culture, Differentiations, and Preparation
  22. SOP for iPSC-CFs
  23. SOP for Live Dead Assay
  24. SOP for Microfluidics
  25. SOP for PolyHEMA Coating of Glass Slides
  26. SOP for primers, RNA, cDNA, and qPCR
  27. SOP for TMSPMA Treatment of Glass Slides
  28. SOP for AIM Biotech Chip Coating
  29. SOP for IF