{"id":2745,"date":"2022-09-15T12:03:58","date_gmt":"2022-09-15T19:03:58","guid":{"rendered":"https:\/\/faculty.engineering.asu.edu\/nikkhah\/?page_id=2745"},"modified":"2022-09-21T11:07:48","modified_gmt":"2022-09-21T18:07:48","slug":"lab-sops","status":"publish","type":"page","link":"https:\/\/faculty.engineering.asu.edu\/nikkhah\/lab-sops\/","title":{"rendered":"Lab Standard Operating Procedures"},"content":{"rendered":"\n

General Lab Protocols<\/h1>\n\n\n\n
  1. Ordering Non-Catalog Items<\/a><\/li>
  2. Ordering through Sunrise<\/a><\/li>
  3. Ordering via Purchase Order<\/a><\/li>
  4. Heart-on-Chip Training Presentation<\/a><\/li><\/ol>\n\n\n\n

    Equipment Protocols<\/h1>\n\n\n\n
    1. SOP for Autoclaving Pipette Tips<\/a><\/li>
    2. SOP for Operating Biosafety Cabinet – Level 2<\/a><\/li><\/ol>\n\n\n\n

      Technical Protocols<\/h1>\n\n\n\n
      1. Biotium SOP for Live Dead Assay<\/a><\/li>
      2. BMSEED User Manual for the Electrophysiology Module<\/a><\/li>
      3. BMSEED User Manual for the Electrophysiology Module using the IntanRHX Software<\/a><\/li>
      4. BMSEED User Manual for the Temperature Controller<\/a><\/li>
      5. OD versus Concentration for Gold Nanorods<\/a><\/li>
      6. Optimization of Surface Treatment of sMEAS for seeding hiPSC-CMs<\/a><\/li>
      7. SOP for Actin Staining<\/a><\/li>
      8. SOP for Agarose Microwells – Casting, Equilibrating, and Seeding 3D Petri Dish<\/a><\/li>
      9. SOP for Cancer Invasion Platform<\/a><\/li>
      10. SOP for Cardiac Cell Isolation<\/a><\/li>
      11. SOP for Culturing MDA 231 Cells<\/a><\/li>
      12. SOP for Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt\/beta-catenin signaling<\/a><\/li>
      13. SOP for Fabricating Hydrogels<\/a><\/li>
      14. SOP for Freezing hCF<\/a><\/li>
      15. SOP for GelMA Synthesis from Porcine Gelatin<\/a><\/li>
      16. SOP for Gold Nanorod Synthesis<\/a><\/li>
      17. SOP for Gold Nanosphere Synthesis<\/a><\/li>
      18. SOP for Gold Nanowire Synthesis<\/a><\/li>
      19. SOP for hiPSC Culture, Differentiation, and Preparation<\/a><\/li>
      20. SOP for Immunostaining of Microfluidic Devices<\/a><\/li>
      21. SOP for IMR90-4 hiPSC Culture, Differentiations, and Preparation<\/a><\/li>
      22. SOP for iPSC-CFs<\/a><\/li>
      23. SOP for Live Dead Assay<\/a><\/li>
      24. SOP for Microfluidics<\/a><\/li>
      25. SOP for PolyHEMA Coating of Glass Slides<\/a><\/li>
      26. SOP for primers, RNA, cDNA, and qPCR<\/a><\/li>
      27. SOP for TMSPMA Treatment of Glass Slides<\/a><\/li>
      28. SOP for AIM Biotech Chip Coating<\/a><\/li>
      29. SOP for IF<\/a><\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"

        General Lab Protocols Ordering Non-Catalog Items Ordering through Sunrise Ordering via Purchase Order Heart-on-Chip Training Presentation Equipment Protocols SOP for Autoclaving Pipette Tips SOP for Operating Biosafety Cabinet – Level 2 Technical Protocols Biotium SOP […]<\/p>\n","protected":false},"author":206,"featured_media":0,"parent":0,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2745","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/faculty.engineering.asu.edu\/nikkhah\/wp-json\/wp\/v2\/pages\/2745","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/faculty.engineering.asu.edu\/nikkhah\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/faculty.engineering.asu.edu\/nikkhah\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/faculty.engineering.asu.edu\/nikkhah\/wp-json\/wp\/v2\/users\/206"}],"replies":[{"embeddable":true,"href":"https:\/\/faculty.engineering.asu.edu\/nikkhah\/wp-json\/wp\/v2\/comments?post=2745"}],"version-history":[{"count":0,"href":"https:\/\/faculty.engineering.asu.edu\/nikkhah\/wp-json\/wp\/v2\/pages\/2745\/revisions"}],"wp:attachment":[{"href":"https:\/\/faculty.engineering.asu.edu\/nikkhah\/wp-json\/wp\/v2\/media?parent=2745"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}