The diversity and unique physiochemical properties of 2D materials allow for a wide range of biological and biomedical applications. Most research on these exciting materials have been focused on exploring the synthesis, properties, and potential applications. The inherent toxicity and biocompatibility need to be investigated before further exploration and development of these materials into impactful products. Our mission is to understand the cell viability and toxicity of 2D quantum materials. In our studies we use fluorescence imaging coupled with quantitative data from the cell viability measurements can paint a more complete picture of the cell’s toxicity with 2D materials. Some of the example target materials include transition metal dichalcogenides (MoS2, Janus MoSSe, MoTe2) as well as other quantum materials including Bi2Te3, NbTe2, HfTe5 etc.